It is an immunologic phenomenon whereby the effectiveness of antibodies to form immune complexes can be impaired when concentrations of an antibody or an antigen are very high.

What is the Hook Effect? The Hook Effect, also known as the prozone or High Dose Hook Effect, occurs when extremely high concentrations of the target analyte cause a paradoxical decrease in signal …

Hook effect is defined as a phenomenon in immunoassays where a very high concentration of an analyte leads to a falsely lowered observed value due to the formation of single antibody-analyte …

Oct 7, 2014 · The hook effect is one of the most widely recognized limitations of immunoassays. It gives falsely low results with certain immunoassays in the presence of excess amount of analyte of interest.

Dec 1, 2020 · The main aim of this work is to identify and understand these high antigen concentration effects in order to develop ubiquitous strategies to interpret and mitigate such effects.

Nov 13, 2020 · To unravel the hook effect, we devised an inexpensive, dynamic, smartphone-based method for directly identifying the concentration-dependent effects across three different sandwich …

Dec 3, 2025 · The Hook Effect is a phenomenon where an extremely high concentration of the substance being measured, known as the analyte, leads to a misleadingly low or even negative test …

The "hook effect" (or "hooking effect") is a phenomenon common to bimolecular detection systems involving saturable reagents (beads, labeled antibodies, streptavidin, etc.) used to capture specific …

The hook effect or the prozone effect is an immunologic phenomenon whereby the effectiveness of antibodies to form immune complexes is sometimes impaired when concentrations of an antibody or …

Monoclonal antibodies (MAB’s) - Monoclonals specific antigen (epitope) (Figure 3). Careful high degree of sensitivity and specificity against can bind to only one type of epitope on the surface coating. The …